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Sterilisation - D-Wert, F-Wert, z-Wert, Letalitätswert, ...
#3
Hallo Laganon,

jajaja, es besteht noch Bedarf!


Aaaalso: ich habe einen Leitkeim, eine Sterilisationstemperatur, D-Wert und z-Wert zur Sterilisationstemperatur für diesen Keim.


Ausgangsparameter:
Leitkeim: B.subtilis
Sterilisationstemperatur T=115°C
D-Wert (115°C) = 2.2
z-Wert (115°C) = 8-13
Ausgangskeimgehalt = 10^06
Zielkeimgehalt = 10^-12


Berechnung F-Wert
F-Wert = D-Wert * (log Ausgangskeimgehalt - log Zielkeimgehalt) = 2.2 * (log 10^06 - log 10^-12) = 39.6 Minuten = 40 Minuten


Soweit alles klar, danach komme ich nicht klar wie ich den z-Wert und die Letalrate einrechne:

Ich habe 2 z-Werte in der Literatur gefunden, 8 und 13.

Letalrate "1" = t * 10^((121°c-115°C)/z-Wert) = 1 Minute * 10^((121°C-115°C)/8) = 0.177   bzw.
Letalrate "2" = t * 10^((121°c-115°C)/z-Wert) = 1 Minute * 10^((121°C-115°C)/13) = 0.345



Und weiter? Wie lange muss ich nun sterilisieren? 


Klar habe ich das in der Theorie mal im Studium gelernt und ein ehemaliger Kollege hat mir auch mal geholfen bei den ersten Berechnungen, aber in seinen mir vorliegenden Notizen ist irgendwo ein Schritt nicht aufgeschrieben und ich habe leider keine Möglichkeit ihn zu fragen und stolpere und stolpere und komme irgendwie nicht weiter, auch Lesen hat nicht sonderlich weitergeholfen. 


1000 Dank fürs Mitdenken!!! 

LG
Saftschubse
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RE: Sterilisation - D-Wert, F-Wert, z-Wert, Letalitätswert, ... - von Saftschubse - 26.09.2016, 16:49

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